Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-96707 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE1L2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MEGSEPVAAH QGEEASCSSW GTGSTNKNLP IMSTASVEID DALYSRQRYV LGDTAMQKMA KSHVFLSGMG GLGLEIAKNL VLAGIKAVTI HDTEKCQAWD LGTNFFLSED DVVNKRNRAE AVLKHIAELN PYVHVTSSSV PFNETTDLSF LDKYQCVVLT EMKLPLQKKI NDFCRSQCPP IKFISADVHG IWSRLFCDFG DEFEVLDTTG EEPKEIFISN ITQANPGIVT CLENHPHKLE TGQFLTFREI NGMTGLNGSI; Positive Samples: U-251MG, SKOV3, 293T, Mouse brain
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.78 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of UBE1L2 in A549 cells. Samples were incubated with UBE1L2 Polyclonal antibody (Product # PA5-96707). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of UBE1L2 in 200 μg extracts of 293T cells. Samples were precipitated with 3 μg UBE1L2 Polyclonal antibody (Product # PA5-96707). Western blot was performed from the immunoprecipitate using UBE1L2 Polyclonal antibody (Product # PA5-96707) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of UBE1L2 was performed in 200 µg extracts of 293T cells using UBE1L2 Polyclonal Antibody (Product # PA5-96707). Western blot was performed from the immunoprecipitate using UBE1L2 Polyclonal Antibody.