13-5889-80
antibody from Invitrogen Antibodies
Targeting: ICOSLG
B7-H2, B7h, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, KIAA0653
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13-5889-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD275 (B7-H2) Monoclonal Antibody (MIH12), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway. Applications Reported: This MIH12 antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections. Applicability of MIH12 for immunoprecipitation and immunoblotting (WB) has not been determined. Applications Tested: The MIH12 antibody has been tested by flow cytometric analysis of normal human peripheral blood monocyte-derived dendritic cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- MIH12
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors.
Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.
Ventura Ferreira MS, Bienert M, Müller K, Rath B, Goecke T, Opländer C, Braunschweig T, Mela P, Brümmendorf TH, Beier F, Neuss S
Stem cell research & therapy 2018 Feb 5;9(1):28
Stem cell research & therapy 2018 Feb 5;9(1):28
Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.
Sakthivel P, Grunewald J, Eklund A, Bruder D, Wahlström J
Clinical and experimental immunology 2016 Feb;183(2):294-306
Clinical and experimental immunology 2016 Feb;183(2):294-306
Tumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors.
Pedroza-Gonzalez A, Zhou G, Vargas-Mendez E, Boor PP, Mancham S, Verhoef C, Polak WG, Grünhagen D, Pan Q, Janssen H, Garcia-Romo GS, Biermann K, Tjwa ET, IJzermans JN, Kwekkeboom J, Sprengers D
Oncoimmunology 2015 Jun;4(6):e1008355
Oncoimmunology 2015 Jun;4(6):e1008355
Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues.
Youngnak-Piboonratanakit P, Tsushima F, Otsuki N, Igarashi H, Omura K, Azuma M
Scandinavian journal of immunology 2006 Mar;63(3):191-8
Scandinavian journal of immunology 2006 Mar;63(3):191-8
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD275 (B7-H2) PE (filled histogram). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
- Conjugate
- Biotin