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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- BAF1139 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Siglec-9 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Siglec-9 in direct ELISAs and Western blots. In direct ELISAs, less than 0.2% cross-reactivity with recombinant human (rh) Siglec-2, rhSiglec-3, rhSiglec-5, rhSiglec-7, and rhSiglec-10 is observed. In Western blots, approximately 25% cross-reactivity with rhSiglec-7 is observed, approximately 5% cross-reactivity with rhSiglec-3 and rhSiglec-5 is observed, and less than 1% cross-reactivity with rhSiglec-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Siglecs facilitate HIV-1 infection of macrophages through adhesion with viral sialic acids.
Zou Z, Chastain A, Moir S, Ford J, Trandem K, Martinelli E, Cicala C, Crocker P, Arthos J, Sun PD
PloS one 2011;6(9):e24559
PloS one 2011;6(9):e24559
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Siglec-9 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human Siglec-9 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1139, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
