Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- AF1139 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Siglec-9 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Siglec-9 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) Siglec-7 is observed, 5% cross-reactivity with rhSiglec-3 and rhSiglec-5 is observed, and less than 1% cross-reactivity with rhSiglec-2 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y336
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Increased expression of Siglec-9 in chronic obstructive pulmonary disease.
Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs.
Zeng Z, Li M, Wang M, Wu X, Li Q, Ning Q, Zhao J, Xu Y, Xie J
Scientific reports 2017 Aug 31;7(1):10116
Scientific reports 2017 Aug 31;7(1):10116
Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 11;35(6):2452-64
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 11;35(6):2452-64
Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs.
Ando M, Tu W, Nishijima K, Iijima S
Biochemical and biophysical research communications 2008 May 9;369(3):878-83
Biochemical and biophysical research communications 2008 May 9;369(3):878-83
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Siglec-9 in Human PBMCs. Siglec-9 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Siglec-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1139) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.