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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- H00008195-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008195-D01, RRID:AB_10658897
- Product name
- MKKS MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human MKKS protein.
- Antigen sequence
MSRLEAKKPSLCKSEPLTTERVRTTLSVLKRIVTS
CYGPSGRLKQLHNGFGGYVCTTSQSSALLSHLLVT
HPILKILTASIQNHVSSFSDCGLFTAILCCNLIEN
VQRLGLTPTTVIRLNKHLLSLCISYLKSETCGCRI
PVDFSSTQILLCLVRSILTSKPACMLTRKETEHVS
ALILRAFLLTIPENAEGHIILGKSLIVPLKGQRVI
DSTVLPGILIEMSEVQLMRLLPIKKSTALKVALFC
TTLSGDTSDTGEGTVVVSYGVSLENAVLDQLLNLG
RQLISDHVDLVLCQKVIHPSLKQFLNMHRIIAIDR
IGVTLMEPLTKMTGTQPIGSLGSICPNSYGSVKDV
CTAKFGSKHFFHLIPNEATICSLLLCNRNDTAWDE
LKLTCQTALHVLQLTLKEPWALLGGGCTETHLAAY
IRHKTHNDPESILKDDECTQTELQLIAEAFCSALE
SVVGSLEHDGGEILTDMKYGHLWSVQADSPCVANW
PDLLSQCGCGLYNSQEELNWSFLRSTRRPFVPQSC
LPHEAVGSASNLTLDCLTAKLSGLQVAVETANLIL
DLSYVIEDKN- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Combining Cep290 and Mkks ciliopathy alleles in mice rescues sensory defects and restores ciliogenesis.
Rachel RA, May-Simera HL, Veleri S, Gotoh N, Choi BY, Murga-Zamalloa C, McIntyre JC, Marek J, Lopez I, Hackett AN, Zhang J, Brooks M, den Hollander AI, Beales PL, Li T, Jacobson SG, Sood R, Martens JR, Liu P, Friedman TB, Khanna H, Koenekoop RK, Kelley MW, Swaroop A
The Journal of clinical investigation 2012 Apr;122(4):1233-45
The Journal of clinical investigation 2012 Apr;122(4):1233-45
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MKKS MaxPab rabbit polyclonal antibody. Western Blot analysis of MKKS expression in A-431.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MKKS expression in transfected 293T cell line (H00008195-T02) by MKKS MaxPab polyclonal antibody.Lane 1: MKKS transfected lysate(62.30 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of MKKS transfected lysate using anti-MKKS MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MKKS purified MaxPab mouse polyclonal antibody (B01P) (H00008195-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
