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- References [2]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ABIN359486 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Eukaryotic Translation Initiation Factor 4A1 (EIF4A1) (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide peptide between 1~30 amino acids from the N-terminalregion of Human EIF4A1. Genename: EIF4A1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Submitted references PDCD4 inhibits translation initiation by binding to eIF4A using both its MA3 domains.
Anti-inflammatory lipid mediator 15d-PGJ2 inhibits translation through inactivation of eIF4A.
Suzuki C, Garces RG, Edmonds KA, Hiller S, Hyberts SG, Marintchev A, Wagner G
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 4;105(9):3274-9
Proceedings of the National Academy of Sciences of the United States of America 2008 Mar 4;105(9):3274-9
Anti-inflammatory lipid mediator 15d-PGJ2 inhibits translation through inactivation of eIF4A.
Kim WJ, Kim JH, Jang SK
The EMBO journal 2007 Dec 12;26(24):5020-32
The EMBO journal 2007 Dec 12;26(24):5020-32
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Left: Western blot analysis of EIF4A1 antibody Cat.-No AP13970PU-N (N-term) in K562 cell line lysates (35 μg/lane). EIF4A1 (arrow) was detected using the purified antibody. Right: Western blot analysis of EIF4A1 (arrow) using EIF4A1 antibody Cat.-No AP13970PU-N (N-term). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-Fixed, Paraffin-Eembedded Human lung carcinoma tissue stained with EIF4A1 antibody Cat.-No AP13970PU-N (N-term),which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
