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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- MA5-51507 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF4A1 Recombinant Rabbit Monoclonal Antibody (3T7U10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 3T7U10
- Vial size
- 100 µL
- Concentration
- 0.079 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of eIF4A1 in 293T cells. Samples were incubated with eIF4A1 Recombinant monoclonal antibody (Product # MA5-51507) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of eIF4A1 in C6 cells. Samples were incubated with eIF4A1 Recombinant monoclonal antibody (Product # MA5-51507) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of eIF4A1 in NIH/3T3 cells. Samples were incubated with eIF4A1 Recombinant monoclonal antibody (Product # MA5-51507) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of eIF4A1 in 300 µg extracts of HepG2 cells. Samples were incubated with eIF4A1 Recombinant monoclonal antibody (Product # MA5-51507). Western blot was performed from the immunoprecipitate using eIF4A1 Rabbit mAb (Product # MA5-51507) at a dilution of 1:71,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF4A1 in paraffin-embedded mouse intestine. Samples were incubated with eIF4A1 Recombinant monoclonal antibody (Product # MA5-51507) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF4A1 in paraffin-embedded mouse liver. Samples were incubated with eIF4A1 Recombinant monoclonal antibody (Product # MA5-51507) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
