RQ5520

antibody from NSJ Bioreagents

Targeting: EIF4A1 DDX2A, EIF-4A, EIF4A

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5520

Provider

NSJ Bioreagents

Product name

EIF4A1/2/3 Antibody

Antibody type

Polyclonal

Description

This highly specific EIF4A1/2/3 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the EIF4A1/2/3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta 2) HEK293, 3) A549, 4) HepG2, 5) U-87 MG, 6) PC-3 and 7) K562 lysate with EIF4A1/2/3 antibody. Predicted molecular weight ~46 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat thymus, 2) mouse thymus, 3) mouse lung and 7) mouse SP20 lysate with EIF4A1/2/3 antibody. Predicted molecular weight ~46 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with EIF4A1/2/3 antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human colon cancer with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human lung cancer with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse intestine with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine with EIF4A1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human U-87 MG cells with EIF4A1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF4A1/2/3 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with EIF4A1/2/3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= EIF4A1/2/3 antibody.