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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MA1-91683 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-M-cadherin Monoclonal Antibody (21G4)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA1-91683 detects M Cadherin from human samples. MA1-91683 has been successfully used in immunofluorescence, Western blot applications. The MA1-91683 immunogen is: Recombinant full length protein - the extracellular domain of human M-Cadherin Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 21G4
- Vial size
- 50 µg
- Concentration
- 2.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot of M Cadherin in whole cell lysates from COS-7 (lane 1) and human M-cadherin overexpressed in COS-7 cells (lane 2). Samples were separated on SDS-PAGE, transferred to PVDF membrane, and probed using a M Cadherin monoclonal antibody (Product # MA1-91683) at a dilution of 0.5µg/mL for 1 hour at room temperature, followed by ECL detection at an exposure time of 30 seconds.
