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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00011PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM00011PU-N, RRID:AB_973471
- Product name
- anti Cadherin-15 (Extracell. Dom.)
- Antibody type
- Monoclonal
- Antigen
- Recombinant extracellular domain of Murine M-Cadherin
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 12G4
- Vial size
- 0.1 mg
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Detection of M-Cadherin: Whole cell lysates were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MCAD-12G4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: 10T1/2, Lane 2: Mouse M-Cadherin overexpressed in 10T1/2 cells.
