Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00009607-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00009607-M01, RRID:AB_1136906
- Product name
- CART monoclonal antibody (M01), clone 3E4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant CART.
- Antigen sequence
MESSRVRLLPLLGAALLLMLPLLGTRAQEDAELQP
RALDIYSAVDDASHEKELIEALQEVLKKLKSKRVP
IYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRG
TSCNSFLLKCL- Isotype
- IgG
- Antibody clone number
- 3E4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CART expression in transfected 293T cell line by CART monoclonal antibody (M01), clone 3E4.Lane 1: CART transfected lysate(12.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CARTPT is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol