H00009607-M01

antibody from Abnova Corporation

Targeting: CARTPT CART

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00009607-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00009607-M01, RRID:AB_1136906

Product name

CART monoclonal antibody (M01), clone 3E4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant CART.

Antigen sequence

MESSRVRLLPLLGAALLLMLPLLGTRAQEDAELQP
RALDIYSAVDDASHEKELIEALQEVLKKLKSKRVP
IYEKKYGQVPMCDAGEQCAVRKGARIGKLCDCPRG
TSCNSFLLKCL


Isotype

IgG

Antibody clone number

3E4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CART expression in transfected 293T cell line by CART monoclonal antibody (M01), clone 3E4.Lane 1: CART transfected lysate(12.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CARTPT is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol