Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [11]
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Validation data
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- Product number
- AMAb90852 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#AMAb90852, RRID:AB_2665691
- Product name
- Anti-RHOT1
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
THIVDYSEAEQSDEQLHQEISQANVICIVYAVNNK
HSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDL
VEYSSMETILPIMNQYTEIE- Epitope
- Binds to an epitope located within the peptide sequence VTSRWIPLIN as determined by overlapping synthetic peptides.
- Isotype
- IgG
- Antibody clone number
- CL1083
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
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- Experimental details
- Lane 1: Marker [kDa]Lane 2: Human cerebral cortex
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- Atlas Antibodies (provider)
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- Experimental details
- Western blot analysis in human cerebral cortex tissue.
Supportive validation
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human cerebellum shows strong positivity in neuropil of the molecular and granular cell layers, as well as in Purkinje cells.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong immunoreactivity in the neuropil.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human small intestine shows cytoplasmic immunoreactivity in glandular cells.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human testis shows strong granular cytoplasmic immunoreactivity in seminiferous tubules cells.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human breast cancer shows moderate cytoplasmic positivity in tumor cells.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human stomach cancer shows cytoplasmic immunoreactivity in tumor cells.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.
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- Atlas Antibodies (provider)
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- Experimental details
- Immunohistochemical staining of human skeletal muscle shows weak cytoplasmic positivity in striated muscle fibers.