Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- MA5-24622 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RHOT1 Monoclonal Antibody (CL1083)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: THIVDYSEAE QSDEQLHQEI SQANVICIVY AVNNKHSIDK VTSRWIPLIN ERTDKDSRLP LILVGNKSDL VEYSSMETIL PIMNQYTEIE Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%. Binds to an epitope located within the peptide sequence VTSRWIPLIN as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL1083
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RHOT1 in human cerebral cortex tissue using a RHOT1 Monoclonal Antibody (CL1083) (Product # MA5-24622).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RHOT1 in human cerebral cortex using RHOT1 Monoclonal Antibody (CL1083) (Product # MA5-24622) shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RHOT1 in human testis using RHOT1 Monoclonal Antibody (CL1083) (Product # MA5-24622) shows moderate cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RHOT1 in human heart muscle using RHOT1 Monoclonal Antibody (CL1083) (Product # MA5-24622) shows strong cytoplasmic positivity in cardiomyocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RHOT1 in human skeletal muscle using RHOT1 Monoclonal Antibody (CL1083) (Product # MA5-24622) shows weak cytoplasmic positivity in striated muscle fibers.