Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [5]
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- Product number
- NBP1-89011 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89011, RRID:AB_11034143
- Product name
- Rabbit Polyclonal Rhot1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Rhot1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling.
Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.
Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.
The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis.
Stephen TL, Higgs NF, Sheehan DF, Al Awabdh S, López-Doménech G, Arancibia-Carcamo IL, Kittler JT
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Dec 2;35(48):15996-6011
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Dec 2;35(48):15996-6011
Mitotic redistribution of the mitochondrial network by Miro and Cenp-F.
Kanfer G, Courthéoux T, Peterka M, Meier S, Soste M, Melnik A, Reis K, Aspenström P, Peter M, Picotti P, Kornmann B
Nature communications 2015 Aug 11;6:8015
Nature communications 2015 Aug 11;6:8015
Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.
Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT
The Journal of biological chemistry 2014 May 23;289(21):14569-82
The Journal of biological chemistry 2014 May 23;289(21):14569-82
The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy.
Bingol B, Tea JS, Phu L, Reichelt M, Bakalarski CE, Song Q, Foreman O, Kirkpatrick DS, Sheng M
Nature 2014 Jun 19;510(7505):370-5
Nature 2014 Jun 19;510(7505):370-5
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis.
De Vos KJ, Mórotz GM, Stoica R, Tudor EL, Lau KF, Ackerley S, Warley A, Shaw CE, Miller CC
Human molecular genetics 2012 Mar 15;21(6):1299-311
Human molecular genetics 2012 Mar 15;21(6):1299-311
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human cerebellum shows positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human cerebral cortex shows positivity in neuronal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human liver shows positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human pancreas shows positivity in exocrine glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Rhot1 Antibody [NBP1-89011] - Staining of human small intestine shows positivity in glandular cells.