HPA010687

antibody from Atlas Antibodies

Targeting: RHOT1 ARHT1, FLJ11040, MIRO-1, MIRO1

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA010687

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA010687, RRID:AB_1079813

Product name

Anti-RHOT1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human RHOT1, Gene description: ras homolog family member T1, Alternative Gene Names: ARHT1, FLJ11040, MIRO-1, Validated applications: IHC, Uniprot ID: Q8IXI2, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

A mitochondrial inside-out iron-calcium signal reveals drug targets for Parkinson’s disease

Bharat V, Durairaj A, Vanhauwaert R, Li L, Muir C, Chandra S, Kwak C, Le Guen Y, Nandakishore P, Hsieh C, Rensi S, Altman R, Greicius M, Feng L, Wang X
Cell Reports 2023;42(12):113544

---

Mitochondrial dysfunction and mitophagy defects in LRRK2-R1441C Parkinson’s disease models

Williamson M, Madureira M, McGuinness W, Heon-Roberts R, Mock E, Naidoo K, Cramb K, Caiazza M, Malpartida A, Lavelle M, Savory K, Humble S, Patterson R, Davis J, Connor-Robson N, Ryan B, Wade-Martins R
Human Molecular Genetics 2023;32(18):2808-2821

---

Mitochondrial Defects in Fibroblasts of Pathogenic MAPT Patients

Bharat V, Hsieh C, Wang X
Frontiers in Cell and Developmental Biology 2021;9

---

A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidation

Li L, Conradson D, Bharat V, Kim M, Hsieh C, Minhas P, Papakyrikos A, Durairaj A, Ludlam A, Andreasson K, Partridge L, Cianfrocco M, Wang X
Nature Metabolism 2021;3(9):1242-1258

---

Miro1-dependent mitochondrial dynamics in parvalbumin interneurons

Kontou G, Antonoudiou P, Podpolny M, Szulc B, Arancibia-Carcamo I, Higgs N, Lopez-Domenech G, Salinas P, Mann E, Kittler J
eLife 2021;10

---

Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling

Ordureau A, Paulo J, Zhang J, An H, Swatek K, Cannon J, Wan Q, Komander D, Harper J
Molecular Cell 2020;77(5):1124-1142.e10

---

Miro1 Marks Parkinson’s Disease Subset and Miro1 Reducer Rescues Neuron Loss in Parkinson’s Models

Hsieh C, Li L, Vanhauwaert R, Nguyen K, Davis M, Bu G, Wszolek Z, Wang X
Cell Metabolism 2019;30(6):1131-1140.e7

---

Bok regulates mitochondrial fusion and morphology.

Schulman JJ, Szczesniak LM, Bunker EN, Nelson HA, Roe MW, Wagner LE 2nd, Yule DI, Wojcikiewicz RJH
Cell death and differentiation 2019 Dec;26(12):2682-2694

---

Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy

McLelland G, Goiran T, Yi W, Dorval G, Chen C, Lauinger N, Krahn A, Valimehr S, Rakovic A, Rouiller I, Durcan T, Trempe J, Fon E
eLife 2018;7

---

Miro proteins prime mitochondria for Parkin translocation and mitophagy

Safiulina D, Kuum M, Choubey V, Gogichaishvili N, Liiv J, Hickey M, Cagalinec M, Mandel M, Zeb A, Liiv M, Kaasik A
The EMBO Journal 2018;38(2)

---

Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson’s models

Shaltouki A, Hsieh C, Kim M, Wang X
Acta Neuropathologica 2018;136(4):607-620

---

Mechanism and regulation of the Lys6-selective deubiquitinase USP30.

Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
Nature structural & molecular biology 2017 Nov;24(11):920-930

---

Facilitation of axon regeneration by enhancing mitochondrial transport and rescuing energy deficits

Zhou B, Yu P, Lin M, Sun T, Chen Y, Sheng Z
Journal of Cell Biology 2016;214(1):103-119

---

DISC1-dependent Regulation of Mitochondrial Dynamics Controls the Morphogenesis of Complex Neuronal Dendrites.

Norkett R, Modi S, Birsa N, Atkin TA, Ivankovic D, Pathania M, Trossbach SV, Korth C, Hirst WD, Kittler JT
The Journal of biological chemistry 2016 Jan 8;291(2):613-29

---

Mitotic redistribution of the mitochondrial network by Miro and Cenp-F

Kanfer G, Courthéoux T, Peterka M, Meier S, Soste M, Melnik A, Reis K, Aspenström P, Peter M, Picotti P, Kornmann B
Nature Communications 2015;6(1)

---

Miro1 Regulates Activity-Driven Positioning of Mitochondria within Astrocytic Processes Apposed to Synapses to Regulate Intracellular Calcium Signaling

Stephen T, Higgs N, Sheehan D, Al Awabdh S, López-Doménech G, Arancibia-Carcamo I, Kittler J
The Journal of Neuroscience 2015;35(48):15996-16011

---

The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy

Bingol B, Tea J, Phu L, Reichelt M, Bakalarski C, Song Q, Foreman O, Kirkpatrick D, Sheng M
Nature 2014;510(7505):370-375

---

Lysine 27 Ubiquitination of the Mitochondrial Transport Protein Miro Is Dependent on Serine 65 of the Parkin Ubiquitin Ligase

Birsa N, Norkett R, Wauer T, Mevissen T, Wu H, Foltynie T, Bhatia K, Hirst W, Komander D, Plun-Favreau H, Kittler J
Journal of Biological Chemistry 2014;289(21):14569-14582

---

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323

---

VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis

De Vos K, Mórotz G, Stoica R, Tudor E, Lau K, Ackerley S, Warley A, Shaw C, Miller C
Human Molecular Genetics 2012;21(6):1299-1311