13640-1-AP

antibody from Proteintech Group

Targeting: BCAT1 BCT1

Provider product page for 13640-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [55]
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Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 13640-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13640-1-AP, RRID:AB_2063569
Product name: BCAT1 antibody
Antibody type: Polyclonal
Description: KD/KO validated BCAT1 antibody (Cat. #13640-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

BCAT1 protein structure - 13640-1-AP shown in red.

Submitted references Vascular Smooth Muscle Cell-Specific BCAT2 Deficiency Attenuates Diabetic Atherosclerotic Calcification via Histone Propionylation.

Zhang L, Yang Y, Yuan W, Dai Z, Li L, Sun Z, Shao C, Zang G, Qian Y, Wang Z
Research (Washington, D.C.) 2026;9:1052

ZNF33A activates Wnt/β-catenin signaling and promotes BCAA catabolism in colorectal cancer.

Yu W, Chen J, Pu X, Shen H, Cao L, Zhu L, Pei H, Lin X
Cell communication and signaling : CCS 2026 Mar 9;24(1)

Ageing promotes metastasis via activation of the integrated stress response.

Patel AAH, Dzanan JJ, Ali KX, Eklund EA, Alvarez SW, Raj D, Dankis M, Altinönder I, Schwarz M, Le Gal K, Bedel E, El Zowalaty AE, Jonasson E, Albatrok H, Gul N, Bossowski JP, Pillai R, Micke P, Botling J, Akyürek LM, Angeletti D, Sayin SI, Härtlova A, Papagiannakopoulos T, Olofsson Bagge R, Ståhlberg A, Hallqvist A, Wiel C, Sayin VI
Nature 2026 Apr;652(8112):1339-1348

BCAT1-dependent HIF-1α stabilization is a targetable metabolic vulnerability in hepatocellular carcinoma.

Zhang MS, Kwan KK, Tse AP, Wang G, Wei LL, Sun K, Chui NN, Lee D, Bao MH, Pang X, Wu Z, Chen Y, Wang Y, Yang Z, Jiang X, Li Q, Zhang Y, Zhong Y, Cheu JW, Chen Y, Li W, Wong CM, Wang J, Cai Z, Zhang Q, Liang T, Ng IO, Papathanassiu AE, Wong CC
Cell reports. Medicine 2026 Apr 29;:102784

The ACSS2-PPARD-BCAT1 axis synchronously regulates branched-chain amino acid metabolism and development in pancreatic cancer.

Chen H, Ye Z, Liu L, Xu W, Shi Y, Ji S, Xu X, Yu X, Hou S, Qin Y, Zhou C
Acta biochimica et biophysica Sinica 2025 Sep 5;58(4):806-18

Mechanisms of Anemarrhenae Rhizoma in Treating Osteoporosis in Rats: An Integrated Metabolomics and Transcriptomics Analysis.

Wen Y, Huang Z, Hong X, Jiang Q, Han L, Wang Q, Wang J, Shang X, Wu Z, Huang T
Rapid communications in mass spectrometry : RCM 2025 Sep 30;39(18):e10069

Bisphenol A Disrupts Spermatogenesis via Excessive Mitophagy-Driven Ferroptosis.

Zhou Y, Duan H, Wang X, Sheng X, Qi X, Liu B, Chen X, Fang Z, Dong W, Xiao L
Environmental science & technology 2025 Oct 28;59(42):22423-22438

Ginsenoside Ro Targets BCAT1/2 to Suppress Ferroptosis: A Novel Therapeutic Strategy for Ischemic Cardioprotection.

Liu J, Wang F, Bai J, Qu S, Tao S, Liu C, Wang Q, Ding W, Lai Q, Zhang J
Journal of agricultural and food chemistry 2025 Oct 22;73(42):26683-26699

Modified Xiaoyaosan rescues depression-like behavior via remodeling gut microbiota and leucine metabolism.

Zhao Y, Wang Q, Wu Z, Zhou Y, Gao X, Gong W, Qin X, Ren Y, Tian J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2025 Nov;147:157241

BCAT1 Activation Reprograms Branched-Chain Amino Acid Metabolism and Epigenetically Promotes Inflammation in Diabetic Retinopathy.

Wang J, Yin Z, Yang J, Sun S, Tang X, Zhang S, Wang YP, Wang H
Investigative ophthalmology & visual science 2025 Jun 2;66(6):59

A Reproducibility Focused Meta-Analysis Method for Single-Cell Transcriptomic Case-Control Studies Uncovers Robust Differentially Expressed Genes.

Nakatsuka N, Adler D, Jiang L, Hartman A, Cheng E, Klann E, Satija R
bioRxiv : the preprint server for biology 2025 Jun 11;

Global analysis of endogenous protein disorder in cells.

Zhang S, Owyong TC, Sanislav O, Englmaier L, Sui X, Wang G, Greening DW, Williamson NA, Villunger A, White JM, Heras B, Wong WWH, Fisher PR, Hong Y
Nature methods 2025 Jan;22(1):124-134

A novel necroptosis-related miRNA signature for predicting the prognosis of esophageal cancer and immune infiltration analysis.

Zhang M, Song S, Wang B, Shang Y, Liu P, Li J
Translational cancer research 2025 Feb 28;14(2):949-965

NR4A1 deficiency promotes carotid plaque vulnerability by activating integrated stress response via targeting Bcat1.

Chen L, Shi Y, Xiao D, Huang Y, Jiang Y, Liang M, Liang F, Xue J, Chen H, Liu Z, Wang X, Zhuang F, Zhou G, Huo H, Cai Z, Shao Q, He B
Cellular and molecular life sciences : CMLS 2025 Feb 22;82(1):91

Irf4 participates in benzene-induced hematopoietic senescence through mitochondrial ROS-dependent BCAA catabolism.

Liu Z, Wang J, Zhang W, Kang H, Huang D, Wang Z, Jiang G, Gao A
Toxicology 2025 Dec;518:154271

Cu-doped dendritic biodegradable nanoplatforms for augmenting cuproptosis and tumor-starvation therapy through mitochondrial metabolic cascade modulation.

Shi H, Xie Z, Zhang J, Lu F, Luo H, Guo P, Jiang M, Weng Z, Luo X, Chen B, Huang H, Teng T
Materials today. Bio 2025 Dec;35:102477

A study on the mechanism by which BCAT1 influences the formation of intracranial aneurysms by regulating autophagy through endoplasmic reticulum stress.

Liu S, Guan H, Wang F
International immunopharmacology 2025 Dec 10;167:115667

m6A-mediated regulation of ECA39 promotes renal fibrosis in chronic kidney disease by enhancing glycolysis and epithelial-mesenchymal transition.

Tong Z, Zhao H, Cui C, Hong M, Ma Y, Sui L, Wang J, Yuan Q, Sun L
Biochimica et biophysica acta. Molecular cell research 2025 Aug;1872(6):119981

Improving reproducibility of differentially expressed genes in single-cell transcriptomic studies of neurodegenerative diseases through meta-analysis.

Nakatsuka N, Adler D, Jiang L, Hartman A, Cheng E, Klann E, Satija R
Nature communications 2025 Aug 12;16(1):7436

SIRT5-mediated BCAT1 desuccinylation and stabilization leads to ferroptosis insensitivity and promotes cell proliferation in glioma.

Wang T, Han XH, Chen JJ, Wang X, Zhang Z, Han XJ, Lu Z
Cell death & disease 2025 Apr 7;16(1):261

Identification of HCAR1 as a ferroptosis-related biomarker of gastric cancer based on a novel ferroptosis-related prognostic model and in vitro experiments.

Zhang H, Zhan J, Zhou J, Liu L, He Y, Le Y, Liu W, Zhou L, Liu Y, Xiang X
Carcinogenesis 2025 Apr 3;46(2)

Branch Chain Amino Acid Metabolism Promotes Brain Metastasis of NSCLC through EMT Occurrence by Regulating ALKBH5 activity.

Mao L, Wang L, Lyu Y, Zhuang Q, Li Z, Zhang J, Gu Z, Lu S, Wang X, Guan Y, Xiong J, Wang Y, Mao Y, Yang H, Liu Y
International journal of biological sciences 2024;20(9):3285-3301

Identify BCAT1 plays an oncogenic role and promotes EMT in KIRC via single cell RNA-seq and experiment.

Li S, Guo Y, Zhu G, Sun L, Zhou F
Frontiers in oncology 2024;14:1446324

MiR-320a Acts as a Tumor Suppressor in Somatotroph Pituitary Neuroendocrine Tumors by Targeting BCAT1.

Zhao S, Li B, Gao H, Zhang Y
Neuroendocrinology 2024;114(1):14-24

BCAT1 alleviates early brain injury by inhibiting ferroptosis through PI3K/AKT/mTOR/GPX4 pathway after subarachnoid hemorrhage.

Liu N, Li C, Yan C, Yan HC, Jin BX, Yang HR, Jiang GY, Gong HD, Li JY, Ma SJ, Liu HL, Gao C
Free radical biology & medicine 2024 Sep;222:173-186

Branched-chain α-ketoacids aerobically activate HIF1α signalling in vascular cells.

Xiao W, Shrimali N, Vigder N, Oldham WM, Clish CB, He H, Wong SJ, Wertheim BM, Arons E, Haigis MC, Leopold JA, Loscalzo J
Nature metabolism 2024 Nov;6(11):2138-2156

Transcription factor EB reprograms branched-chain amino acid metabolism and promotes pancreatic cancer progression via transcriptional regulation of BCAT1.

Wang T, Hu Q, Li B, Fan G, Jing D, Xu J, Hu Y, Dang Q, Ji S, Zhou C, Zhuo Q, Xu X, Qin Y, Yu X, Li Z
Cell proliferation 2024 Nov;57(11):e13694

Branched chain α-ketoacids aerobically activate HIF1α signaling in vascular cells.

Xiao W, Shrimali N, Oldham WM, Clish CB, He H, Wong SJ, Wertheim BM, Arons E, Haigis MC, Leopold JA, Loscalzo J
bioRxiv : the preprint server for biology 2024 May 30;

CHIP-mediated ubiquitin degradation of BCAT1 regulates glioma cell proliferation and temozolomide sensitivity.

Lu Z, Wang XY, He KY, Han XH, Wang X, Zhang Z, Qu XH, Chen ZP, Han XJ, Wang T
Cell death & disease 2024 Jul 29;15(7):538

Branched-Chain Amino Acid Accumulation Fuels the Senescence-Associated Secretory Phenotype.

Liang Y, Pan C, Yin T, Wang L, Gao X, Wang E, Quang H, Huang D, Tan L, Xiang K, Wang Y, Alexander PB, Li QJ, Yao TP, Zhang Z, Wang XF
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jan;11(2):e2303489

BCKDK loss impairs mitochondrial Complex I activity and drives alpha-synuclein aggregation in models of Parkinson's disease.

Jishi A, Hu D, Shang Y, Wang R, Gunzler SA, Qi X
Acta neuropathologica communications 2024 Dec 21;12(1):198

Branched-chain amino acid transaminase 1 confers EGFR-TKI resistance through epigenetic glycolytic activation.

Zhang T, Pan Z, Gao J, Wu Q, Bai G, Li Y, Tong L, Feng F, Lai M, Liu Y, Song P, Ning Y, Tang H, Luo W, Chen Y, Fang Y, Zhang H, Liu Q, Zhang Y, Wang H, Chen Z, Chen Y, Geng M, Ji H, Zhao G, Zhou H, Ding J, Xie H
Signal transduction and targeted therapy 2024 Aug 15;9(1):216

Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells.

Li C, Shi J, Wei S, Jia H
European journal of medical research 2023 Nov 15;28(1):517

Suppressing Mesenchymal Stromal Cell Ferroptosis Via Targeting a Metabolism-Epigenetics Axis Corrects their Poor Retention and Insufficient Healing Benefits in the Injured Liver Milieu.

Hu G, Cui Z, Chen X, Sun F, Li T, Li C, Zhang L, Guo X, Zhao H, Xia Y, Yan W, Yi W, Fan M, Yang R, Wang S, Tao L, Zhang F
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 May;10(13):e2206439

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression.

Qian L, Li N, Lu XC, Xu M, Liu Y, Li K, Zhang Y, Hu K, Qi YT, Yao J, Wu YL, Wen W, Huang S, Chen ZJ, Yin M, Lei QY
Nature metabolism 2023 Jul;5(7):1159-1173

Branched-chain aminotransferase 1 promotes Schwann cell migration and proliferation to accelerate facial nerve regeneration through the Twist/FoxC1 and Sox2 pathways.

Chen Z, Tang Y, Zhu W, Wang H, Cai X, Shen Y, Wang B, Zhao H, Zhu J, Li S
International journal of biological macromolecules 2023 Jul 1;242(Pt 2):124870

Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts.

Ogawa T, Kouzu H, Osanami A, Tatekoshi Y, Sato T, Kuno A, Fujita Y, Ino S, Shimizu M, Toda Y, Ohwada W, Yano T, Tanno M, Miki T, Miura T
Physiological reports 2023 Feb;11(4):e15608

Differential expression pattern, bioinformatics analysis, and validation of circRNA and mRNA in patients with arteriosclerosis.

Liu Y, Wang K, Li G, Chen Z
Frontiers in cardiovascular medicine 2022;9:942797

The proatherosclerotic function of BCAT1 in atherosclerosis development of aged-apolipoprotein E-deficient mice.

Tan L, Lu J, Zhang C, Meng L, Zhu Q
Biochemical and biophysical research communications 2022 Nov 26;631:93-101

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.

Wang Y, Luo M, Wang F, Tong Y, Li L, Shu Y, Qiao K, Zhang L, Yan G, Liu J, Ji H, Xie Y, Zhang Y, Gao WQ, Liu Y
Nature communications 2022 Nov 24;13(1):7215

Impaired expression of BCAT1 relates to muscle atrophy of mouse model of sarcopenia.

Ouyang H, Gao X, Zhang J
BMC musculoskeletal disorders 2022 May 13;23(1):450

Ruscogenin Alleviates Myocardial Ischemia-Induced Ferroptosis through the Activation of BCAT1/BCAT2.

Fu F, Lai Q, Hu J, Zhang L, Zhu X, Kou J, Yu B, Li F
Antioxidants (Basel, Switzerland) 2022 Mar 18;11(3)

Targeting BCAT1 Combined with α-Ketoglutarate Triggers Metabolic Synthetic Lethality in Glioblastoma.

Zhang B, Peng H, Zhou M, Bao L, Wang C, Cai F, Zhang H, Wang JE, Niu Y, Chen Y, Wang Y, Hatanpaa KJ, Copland JA, DeBerardinis RJ, Wang Y, Luo W
Cancer research 2022 Jul 5;82(13):2388-2402

Comprehensive single-cell transcriptional profiling defines shared and unique epithelial injury responses during kidney fibrosis.

Li H, Dixon EE, Wu H, Humphreys BD
Cell metabolism 2022 Dec 6;34(12):1977-1998.e9

BCAT1 Activates PI3K/AKT/mTOR Pathway and Contributes to the Angiogenesis and Tumorigenicity of Gastric Cancer.

Shu X, Zhan PP, Sun LX, Yu L, Liu J, Sun LC, Yang ZH, Ran YL, Sun YM
Frontiers in cell and developmental biology 2021;9:659260

Branched-chain amino acids govern the high learning ability phenotype in Tokai high avoider (THA) rats.

Shida Y, Endo H, Owada S, Inagaki Y, Sumiyoshi H, Kamiya A, Eto T, Tatemichi M
Scientific reports 2021 Nov 29;11(1):23104

BCAT1 overexpression regulates proliferation and c‑Myc/GLUT1 signaling in head and neck squamous cell carcinoma.

Wang H, Wang F, Ouyang W, Jiang X, Wang Y
Oncology reports 2021 May;45(5)

RNA-binding motif protein RBM47 promotes tumorigenesis in nasopharyngeal carcinoma through multiple pathways.

Xu XC, He S, Zhou YQ, Liu CJ, Liu SQ, Peng W, Liu YX, Wei PP, Bei JX, Luo CL
Journal of genetics and genomics = Yi chuan xue bao 2021 Jul 20;48(7):595-605

miR-98-5p inhibits gastric cancer cell stemness and chemoresistance by targeting branched-chain aminotransferases 1.

Zhan P, Shu X, Chen M, Sun L, Yu L, Liu J, Sun L, Yang Z, Ran Y
Life sciences 2021 Jul 1;276:119405

Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.

Zhang B, Chen Y, Shi X, Zhou M, Bao L, Hatanpaa KJ, Patel T, DeBerardinis RJ, Wang Y, Luo W
Cellular and molecular life sciences : CMLS 2021 Jan;78(1):195-206

Oxoeicosanoid receptor inhibition alleviates acute myocardial infarction through activation of BCAT1.

Lai Q, Yuan G, Shen L, Zhang L, Fu F, Liu Z, Zhang Y, Kou J, Liu S, Yu B, Li F
Basic research in cardiology 2021 Jan 23;116(1):3

BCAT1 Overexpression Promotes Proliferation, Invasion, and Wnt Signaling in Non-Small Cell Lung Cancers.

Lin X, Tan S, Fu L, Dong Q
OncoTargets and therapy 2020;13:3583-3594

BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma.

Li JT, Yin M, Wang D, Wang J, Lei MZ, Zhang Y, Liu Y, Zhang L, Zou SW, Hu LP, Zhang ZG, Wang YP, Wen WY, Lu HJ, Chen ZJ, Su D, Lei QY
Nature cell biology 2020 Feb;22(2):167-174

BCAT1 promotes proliferation of endometrial cancer cells through reprogrammed BCAA metabolism.

Wang P, Wu S, Zeng X, Zhang Y, Zhou Y, Su L, Lin W
International journal of clinical and experimental pathology 2018;11(12):5536-5546

BCAT1 expression associates with ovarian cancer progression: possible implications in altered disease metabolism.

Wang ZQ, Faddaoui A, Bachvarova M, Plante M, Gregoire J, Renaud MC, Sebastianelli A, Guillemette C, Gobeil S, Macdonald E, Vanderhyden B, Bachvarov D
Oncotarget 2015 Oct 13;6(31):31522-43

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human kidney tissue were subjected to SDS PAGE followed by western blot with 13640-1-AP(BCAT1 antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The BCAT1/ECA39 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human BCAT1/ECA39. This antibody recognizes human,mouse,rat antigen. The BCAT1/ECA39 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 13640-1-AP.pdf

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