Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP2-01826 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal BCAT1 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.78 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Defect of branched-chain amino acid metabolism promotes the development of Alzheimer's disease by targeting the mTOR signaling.
Li H, Ye D, Xie W, Hua F, Yang Y, Wu J, Gu A, Ren Y, Mao K
Bioscience reports 2018 Aug 31;38(4)
Bioscience reports 2018 Aug 31;38(4)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody. (1:200)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human tonsil using anti-BCAT1 mouse monoclonal antibody.