Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00084197-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00084197-M03, RRID:AB_2188284
- Product name
- Mouse Monoclonal POMK/SGK196 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FLJ23356 - hypothetical protein FLJ23356
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references POMK mutation in a family with congenital muscular dystrophy with merosin deficiency, hypomyelination, mild hearing deficit and intellectual disability.
von Renesse A, Petkova MV, Lützkendorf S, Heinemeyer J, Gill E, Hübner C, von Moers A, Stenzel W, Schuelke M
Journal of medical genetics 2014 Apr;51(4):275-82
Journal of medical genetics 2014 Apr;51(4):275-82
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Western blot analysis of FLJ23356 over-expressed 293 cell line, cotransfected with FLJ23356 Validated Chimera RNAi or non-transfected control. Blot probed with H00084197-M03. GAPDH(36.1 kDa)used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of FLJ23356 expression in transfected 293T cell line by FLJ23356 monoclonal antibody (M03), clone 6F10. Lane 1: FLJ23356 transfected lysatE (38.5 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of FLJ23356 expression in HepG2 (Cat # L019V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of FLJ23356 expression in HeLa(Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: FLJ23356 Antibody (6F10) [H00084197-M03] - Detection limit for recombinant GST tagged FLJ23356 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: FLJ23356 Antibody (6F10) [H00084197-M03] - Analysis of monoclonal antibody to FLJ23356 on HeLa cell. Antibody concentration 10 ug/ml