Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
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Validation data
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- Product number
- GTX629976 - Provider product page
- Provider
- GeneTex
- Product name
- Calnexin antibody [GT1563]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.A. 50 ?g mouse liver lysate/extract7.5 % SDS-PAGECalnexin antibody [GT1563] (GTX629976) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat-2 whole cell lysate/extract7.5 % SDS-PAGECalnexin antibody [GT1563] (GTX629976) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extract7.5 % SDS-PAGECalnexin antibody [GT1563] (GTX629976) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] (GTX629976) diluted at 1:6000.