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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX112886 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112886, RRID:AB_1949822
- Product name
- Calnexin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Huang HJ, Lin CC, Chou HC, Chen YW, Lin ST, Lin YC, Lin DY, Lyu KW, Chan HL
Molecular bioSystems 2014 Dec;10(12):3086-100
Molecular bioSystems 2014 Dec;10(12):3086-100
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:10000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE GTX112886 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX112886 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (15 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX112886) diluted at 1:10000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calnexin antibody detects Calnexin protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Calnexin protein stained by Calnexin antibody (GTX112886) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calnexin(GTX112886) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calnexin antibody detects Calnexin protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Calnexin antibody (GTX112886) diluted at 1:500.
