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- Product number
- NBP2-43765 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Calnexin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Regulation of Osteoclast Growth and Fusion by mTOR/raptor and mTOR/rictor/Akt.
Tiedemann K, Le Nihouannen D, Fong JE, Hussein O, Barralet JE, Komarova SV
Frontiers in cell and developmental biology 2017;5:54
Frontiers in cell and developmental biology 2017;5:54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Analysis of A. 50 ug mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [1563] dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Non-transfected (-) and transfected (+) 293T whole cell extracts (15 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [1563] (NBP2-43765).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody (1563) [NBP2-43765] - Cells cultured without pyruvate were exposed to Akt inhibitor (AI, 5 uM) or rapamycin (1, 10, or 100 nM), and mTOR phosphorylation, total mTOR levels and phosphorylation of p70S6K was assessed, calnexin was used as a protein loading control. The numbers above the blots indicate phospho-S6K levels relative to calnexin. Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fcell.2017.00054/full), licensed under a CC-BY licence.
