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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- V3199 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Calnexin Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- CANX/1541
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the Calnexin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human kidney lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa and 2) MCF7 cell lysate with Calnexin antibody (clone CANX/1541). Predicted molecular weight ~68 kDa but routinely observed at ~90 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human small intestine carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human renal cell carcinoma with Calnexin antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Calnexin Antibody (clone CANX/1541). Confirmation of Integrity and Purity of the Antibody.
