PA5-34804

antibody from Invitrogen Antibodies

Targeting: CANX CNX, IP90, P90

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34804

Provider

Invitrogen Antibodies

Product name

Calnexin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse liver, PC-12, Rat2.

Concentration

0.53 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Calnexin was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Calnexin Polyclonal Antibody (Product # PA5-34804) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Calnexin Polyclonal Antibody (Product # PA5-34804). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. Calnexin Polyclonal Antibody (Product # PA5-34804) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Calnexin Polyclonal Antibody (Product # PA5-34804). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. Lane C: HeLa. Lane D: HepG2 . 7.5% SDS PAGE. Calnexin Polyclonal Antibody (Product # PA5-34804) diluted at 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Calnexin Polyclonal Antibody (Product # PA5-34804). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin Polyclonal Antibody (Product # PA5-34804) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Calnexin was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Calnexin Polyclonal Antibody (Product # PA5-34804) diluted at 1:200. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Calnexin was performed in paraffin-embedded human breast carcinoma tissue using Calnexin Polyclonal Antibody (Product # PA5-34804) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calnexin (Product # PA5-34804) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.