PA5-36733

antibody from Invitrogen Antibodies

Targeting: CANX CNX, IP90, P90

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-36733 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-Calnexin (Ser583) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody detects endogenous protein at a molecular weight of 90 kDa. Purity is >95% by SDS-PAGE.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CANX protein structure - PA5-36733 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Calnexin (Ser583) in Lane 1: A375 whole cell lysate (40 µg), Lane 2: the Lung tissue lysate of mouse (40 µg), Lane 3: PC12 whole cell lysate (40 µg), Lane 4: HEK293T whole cell lysate (40 µg), Lane 5: SGC7901 whole cell lysate (40 µg). Samples were incubated with Phospho-Calnexin (Ser583) polyclonal antibody (Product # PA5-36733) at a dilution of 1:5,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Calnexin (Ser583) in Lane 1: L02 whole cell lysate, Lane 2: HEK293T incubated at 42ºC for 1 hour whole cell lysate, Lane 3: HEK293T whole cell lysate, Lane 4: SGC7901 whole cell lysate, Lane 5: A375 whole cell lysate. Samples were incubated with Phospho-Calnexin (Ser583) polyclonal antibody (Product # PA5-36733) at a dilution of 1:20,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Calnexin (Ser583) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: SGC7901 whole cell lysate (40 µg), Lane 3: A375 whole cell lysate (40 µg). Samples were incubated with Phospho-Calnexin (Ser583) polyclonal antibody (Product # PA5-36733) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Calnexin pSer583 using Phospho-Calnexin pSer583 polyclonal antibody (Product # PA5-36733) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF, Lane 2: Mouse liver tissue lysate, Lane 3: Rat kidney tissue lysate.