MA3-027-A488

antibody from Invitrogen Antibodies

Targeting: CANX CNX, IP90, P90

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-027-A488

Provider

Invitrogen Antibodies

Product name

Calnexin Monoclonal Antibody (AF18), Alexa Fluor™ 488

Antibody type

Monoclonal

Antigen

Other

Description

MA3027A488 detects calnexin from human and mouse tissues. The MA3027A488 antigen is Focus human hepatoma cell lysate.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

AF18

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

A key anti-viral protein, RSAD2/VIPERIN, restricts the release of measles virus from infected cells.

Kurokawa C, Iankov ID, Galanis E
Virus research 2019 Apr 2;263:145-150

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Surf4 (Erv29p) binds amino-terminal tripeptide motifs of soluble cargo proteins with different affinities, enabling prioritization of their exit from the endoplasmic reticulum.

Yin Y, Garcia MR, Novak AJ, Saunders AM, Ank RS, Nam AS, Fisher LW
PLoS biology 2018 Aug;16(8):e2005140

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Effects of an In-Frame Deletion of the 6k Gene Locus from the Genome of Ross River Virus.

Taylor A, Melton JV, Herrero LJ, Thaa B, Karo-Astover L, Gage PW, Nelson MA, Sheng KC, Lidbury BA, Ewart GD, McInerney GM, Merits A, Mahalingam S
Journal of virology 2016 Apr;90(8):4150-4159

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Calnexin (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Calnexin Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA3-027-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Green dye

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 5 Immunofluorescence microscopy of HEK293A cells shows Surf4 accumulates in and around ERESs. (A) Fluorescent signal for endogenous Surf4 (green) was strongest at punctate structures positive for ERES marker Sec23 (red). Note additional Surf4 fluorescence in weblike structures surrounding ERES. (B) HA-Surf4 signal (green) was observed within the ERGIC (ERGIC-53, red). (C) HA-Surf4 (green) showed only low levels of colocalization with cis -Golgi marker, giantin (red). (D) Mutation of proposed COPI recycling motif by replacement of two of three near-carboxy-terminal lysines to alanines (HA-Surf4-AAK, green) increased colocalization with cis -Golgi marker, giantin (red). (E) Newly synthesized HA-Surf4 (green) was found at low levels in the rER (Sec61 marker, red). (F) Surf4 (green) did not colocalize with chaperone, calnexin (red), in the quality control domain. HEK293A cells were transfected with wild-type HA-tagged Surf4 plasmid (B, C, E), or Surf4 KO HEK293A cells were transfected with carboxyl-terminal di-lysine mutation, HA- Surf4 -AAK (D), 18 hr prior to fixation. Bars = 5mum. The cells in each panel are shown 3 times, first with organelle marker, then Surf4 and final panel (with magnified insert) showing overlap. Alexa Fluor secondary antibodies were used for detection. Images were obtained using an LSM 780 (Carl Zeiss) confocal microscope (488 and 561 nm excitation lines; 500-560 and 600-660 nm capture) and Zeiss Axio Imager Z1 with Apotome 2 (single Z stac

Conjugate

Green dye