Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA3-027-A647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calnexin Monoclonal Antibody (AF18), Alexa Fluor™ 647
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA3027A647 detects calnexin from human and mouse tissues. The MA3027A647 antigen is Focus human hepatoma cell lysate.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- AF18
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Calnexin (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Calnexin Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-027-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Red dye
