MA3-027-A647

antibody from Invitrogen Antibodies

Targeting: CANX CNX, IP90, P90

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-027-A647

Provider

Invitrogen Antibodies

Product name

Calnexin Monoclonal Antibody (AF18), Alexa Fluor™ 647

Antibody type

Monoclonal

Antigen

Other

Description

MA3027A647 detects calnexin from human and mouse tissues.

Conjugate

Red dye

Antibody clone number

AF18

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Calnexin (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Calnexin Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA3-027-A647) at a dilution of 2.5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Red dye