Antibody data

Product number
MA3-027 - Provider product page
Provider
Invitrogen Antibodies
Product name
Calnexin Monoclonal Antibody (AF18)
Antibody type
Monoclonal
Antigen
Other
Description
MA3-027 detects calnexin from human and mouse tissues. MA3-027 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunoprecipitation protocols. By Western blot, this antibody detects calnexin in human liver, skin, adrenal gland, heart, colon, testes and ovary extracts. This antibody is not recommended for human kidney or mouse liver lysates in Western blot applications. The predicted molecular weight of this antibody is ~67kDa; however, it may run on SDS-PAGE as an ~90kDA protein. This is due mainly to negative charges at the C-terminus of the protein, which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility. Immunocytochemical staining of calnexin in Huh-7 cells with MA3-027 yields a pattern consistent with specific endoplasmic reticulum staining. The MA3-027 antigen is Focus human hepatoma cell lysate.
Reactivity
Human, Mouse
Host
Mouse
Isotype
IgG
Antibody clone number
AF18
Vial size
100 µL
Concentration
1 mg/mL
Storage
-20° C, Avoid Freeze/Thaw Cycles
CANX protein structure - MA3-027 shown in red.