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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [5]
- Other assay [1]
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- Product number
- MA5-15389 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calnexin Monoclonal Antibody (3H4A7)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-15389 targets Calnexin in indirect ELISA, IF and WB applications and shows reactivity with Human samples. The MA5-15389 immunogen is synthetic peptide corresponding to aa (CEAAEERPWLWVVYILTVAL) of human Calnexin, conjugated to KLH. MA5-15389 detects Calnexin which has a predicted molecular weight of approximately 90kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H4A7
- Vial size
- 100 μL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Plasma extracellular vesicles in people living with HIV and type 2 diabetes are related to microbial translocation and cardiovascular risk.
Vestad B, Nyman TA, Hove-Skovsgaard M, Stensland M, Hoel H, Trøseid AS, Aspelin T, Aass HCD, Puhka M, Hov JR, Nielsen SD, Øvstebø R, Trøseid M
Scientific reports 2021 Nov 9;11(1):21936
Scientific reports 2021 Nov 9;11(1):21936
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- immunofluorescence analysis of HeLa cells using Calnexin monoclonal antibody (Product # MA5-15389) (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- immunofluorescence analysis of HeLa cells using Calnexin monoclonal antibody (Product # MA5-15389) (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- immunofluorescence analysis of HeLa cells using Calnexin monoclonal antibody (Product # MA5-15389) (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- immunofluorescence analysis of HeLa cells using Calnexin monoclonal antibody (Product # MA5-15389) (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- immunofluorescence analysis of HeLa cells using Calnexin monoclonal antibody (Product # MA5-15389) (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Western blot detection of CD9, Hsc70/Hsp70 and Calnexin in plasma pool EVs. As a positive control, lysate from the colorectal cancer cell line SW480 was used. Full-length blots are presented in Supplementary Fig. 3 (Supplementary Information).
