50-0709-42

antibody from Invitrogen Antibodies
Targeting: CD70 CD27L, CD27LG, TNFSF7
Provider product page for 50-0709-42

Antibody data

Product number
50-0709-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD70 Monoclonal Antibody (113-16), eFluor™ 660, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 113-16 monoclonal antibody reacts with human CD70, a type II transmembrane glycoprotein belonging to the TNF superfamily of proteins. CD70 is expressed weakly on macrophages and strongly on activated T, B, NK, and dendritic cells. Expression of CD70 can also be induced on smooth muscle and endothelial cells during inflammation. A ligand of CD27, CD70 plays a role in B cell growth and differentiation into plasma cells. Moreover, this receptor mediates Ig secretion of B cells by plasmacytoid DC. CD70 also plays a role in T cell proliferation and activation, as well as NK cell cytotoxicity. Studies have also shown that CD70 plays a role in regulating Th17 cells. In activated dendritic cells, CD70 helps activate CD8+ T cells and influences their development into memory T cells. The 113-16 antibody has been reported to have antagonist activity. Applications Reported: This 113-16 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 113-16 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
113-16
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD70 protein structure - 50-0709-42 shown in red.