MA5-42758

antibody from Invitrogen Antibodies

Targeting: CD70 CD27L, CD27LG, TNFSF7

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42758

Provider

Invitrogen Antibodies

Product name

CD70 Recombinant Rabbit Monoclonal Antibody (7F4S5)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: Raji, 293T. Immunogen sequence: QRFAQAQQQL PLESLGWDVA ELQLNHTGPQ QDPRLYWQGG PALGRSFLHG PELDKGQLRI HRDGIYMVHI QVTLAICSST TASRHHPTTL AVGICSPASR SISLLRLSFH QGCTIASQRL TPLARGDTLC TNLTGTLLPS RNTDETFFGV QWVRP

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

7F4S5

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD70 was performed using 70% confluent log phase SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD70 Recombinant Rabbit Monoclonal Antibody (7F4S5) (Product # MA5-42758, 1:100) in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ 488 (Product # A27034, 1:2000) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane and secretory localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD70 in Raji cells. Cells were stained with Rabbit IgG isotype control (1 μg/mL, Red line) or CD70 monoclonal antibody (Product # MA5-42758) (1 μg/mL, Blue line), followed by goat anti-Rabbit polyclonal antibody APC (1:200 dilution) staining.