66119-1-IG

antibody from Invitrogen Antibodies

Targeting: PDHA1 PDHA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66119-1-IG

Provider

Invitrogen Antibodies

Product name

PDH E1 alpha Monoclonal Antibody (2B3C10)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: CDLHRLEEG PPVTTVLTRE DGLKYYRMMQ TVRRMELKAD QLYKQKIIRG FCHLCDGQEA CCVGLEAGIN PTDHLITAYR AHGFTFTRGL SVREILAELT GRKGGCAKGK GGSMHMYAKN FYGGNGIVGA QVPLGAGIAL ACKYNGKDEV CLTLYGDGAA NQGQIFEAYN MAALWKLPCI FICENNRYGM GTSVERAAAS TDYYKRGDFI PGLRVDGMDI LCVREATRFA AAYCRSGKGP ILMELQTYRY HGHSMSDPGV SYRTREEIQE VRSKSDPIML LKDRMVNSNL ASVEELKEID VEVRKEIEDA AQFATADPEP PLEELGYHIY SSDPPFEVRG ANQWIKFKSV S (41-390 aa encoded by BC002406)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2B3C10

Vial size

150 µL

Concentration

1.87 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66119-1-IG (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of PDHA1 antibody (66119-1-IG, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 66119-1-IG (PDH E1α antibody) at dilution of 1:100 and Rhodamine-Goat anti-Mouse IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66119-1-IG ( PDH E1α antibody at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66119-1-IG ( PDH E1α antibody at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with .2ug PDH E1α antibody (66119-1-IG, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PDH E1α (IP:66119-1-IG, 3ug; Detection:66119-1-IG 1:10000) with HEK-293 cells lysate 1800ug.