PA5-21536

antibody from Invitrogen Antibodies

Targeting: PDHA1 PDHA

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21536

Provider

Invitrogen Antibodies

Product name

PDHA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, Rat brain. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (91%), Chicken (90%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.39 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Mutations in ARL2BP, a protein required for ciliary microtubule structure, cause syndromic male infertility in humans and mice.

Moye AR, Bedoni N, Cunningham JG, Sanzhaeva U, Tucker ES, Mathers P, Peter VG, Quinodoz M, Paris LP, Coutinho-Santos L, Camacho P, Purcell MG, Winkelmann AC, Foster JA, Pugacheva EN, Rivolta C, Ramamurthy V
PLoS genetics 2019 Aug;15(8):e1008315

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PDHA1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: PDHA1 Polyclonal Antibody (Product # PA5-21536) diluted at 1:2000. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PDHA1 was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: PDHA1 Polyclonal Antibody (Product # PA5-21536) diluted at 1:2000. Blue: Fluoroshield with DAPI.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Pyruvate Dehydrogenase E1 alpha was performed in HepG2 whole cell extracts using 5 µg of PDHA1 Polyclonal Antibody (Product # PA5-21536). Samples were transferred to a membrane and probed with PDHA1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PDHA1 Polyclonal Antibody detects PDHA1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. PDHA1 Polyclonal Antibody (Product # PA5-21536) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Pyruvate Dehydrogenase E1 alpha was performed in HepG2 whole cell extracts using 5 µg of PDHA1 Polyclonal Antibody (Product # PA5-21536). Samples were transferred to a membrane and probed with PDHA1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 6 ARL2BP loss results in impaired sperm tail development. (A) Immunoblot of WT (+/+) and KO (-/-) testis and sperm lysates probed for the indicated sperm tail markers: axonemal markers (Glutamylated tubulin (GT335) and beta-tubulin) and accessory structure markers (Pyruvate dehydrogenase (Pyr. Deh.), Outer Dense Fiber 1 (ODF1), and A-kinase anchoring protein 4 (pre-processed = Pro-AKAP4 and phosphorylated, processed = Ph. AKAP4). Molecular weights in kilodaltons (kDa) are displayed on the left. alpha-tubulin is used as the loading control. (B) WT (+/+) and KO (-/-) murine testes sections stained with PNA lectin (acrosomes, red) and AKAP4 (green), or (C) mitotracker (mitochondria, red) and GT335 (green). The nuclei are stained with DAPI (blue). Scale Bar = 20mum. (D and E) Sperm stained with the indicated sperm tail markers in WT (+/+) and KO (-/-) murine sperm. GT335 -axoneme, green; AKAP4 -fibrous sheath, green; Retinititis Pigmentosa GTPase Regulator (RPGR-axoneme, red); ODF1 -outer dense fibers, green; Heat Shock Protein 60 kDa (HSP60 -mitochondrial sheath, green), and Sperm Flagellar Protein 2 (SPEF2 -axoneme, red). Arrows point to sperm tails. Scale Bar = 10mum.