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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab120952 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- PDHA1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human PDHA1 Antibody, Monoclonal (3H2-F8-B5), could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000 ICC/IF: 1/50-1/200Protein FunctionThe pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 131-389- Antibody clone number
- 3H2-F8-B5
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1,14 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PDHA1 Mouse mAb (Ab120952) - Western Blot All lanes: PDHA1 Mouse mAb (Ab120952) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 41 kDa Exposure time: 20.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- PDHA1 Mouse mAb (Ab120952) - IF/ICC Immunocytochemistry analysis of Pyruvate Dehydrogenase E1 alpha in HeLa using PDHA1 Mouse mAb (Ab120952).
