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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- AMM82964 - Provider product page
- Provider
- EnkiLife Biotech Co., Ltd.
- Product name
- PDHA1 Mouse Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Affinity Purification
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- Monoclonal
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Western blot analysis using PDHA1 mouse mAb against HepG2 (1), HEK293 (2), HL-60 (3), SK-OV-3 (4), PC-3 (5), PANC-1 (6), NRK (7), C2C12 (8), C6 (9) and PC-12 (10) cell lysate.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunoprecipitation using PDHA1 mouse mAb(dilution: 1/250) against HL-60, C2C12, and NRK cell lysate. Western blot analysis using PDHA1 mouse mAb, anti-mouse IgG was used as secondary antibody. Lane 1: cell lysate, Lane 2: Normal Mouse IgG instead of PDHA1 mouse mAb IP in cell lysate, Lane 3: PDHA1 mouse mAb IP in cell lysate.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using PDHA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using PDHA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using PDHA1 mouse mAb with DAB staining.
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Rabbit kidney tissues using ZP2 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using PDHA1 mouse mAb (green) and negative control (red).
- Submitted by
- EnkiLife Biotech Co., Ltd. (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using PDHA1 mouse mAb (green) and negative control (red).
