PA5-117929

antibody from Invitrogen Antibodies

Targeting: EIF3J eIF3-alpha, eIF3-p35, EIF3S1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117929

Provider

Invitrogen Antibodies

Product name

eIF3j Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of eIF3j in A431 cells. Samples were treated with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, incubated with polyclonal antibody (Product # PA5-117929) with a dilution of 1:200 (4°C overnight), followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and DAPI (blue).

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of eIF3j in Lane A: 0.5 mg HeLa Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117929) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 29 kDa , Observed band size : 35 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

eIF3j Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with eIF3j Polyclonal Antibody (Product # PA5-117929) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: eIF3j Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 35 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) of eIF3j in human pancreas. Samples were incubated with polyclonal antibody (Product # PA5-117929) with a dilution of 1:200 .

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of eIF3j in Lane A: 0.5 mg HeLa Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117929) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 29 kDa , Observed band size : 35 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

eIF3j Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with eIF3j Polyclonal Antibody (Product # PA5-117929) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: eIF3j Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 35 kDa.