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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-92155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UGT2B4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LHCKPAKPLP KEMEEFVQSS GENGVVVFSL GSMVSNTSEE RANVIASALA KIPQKVLWRF DGNKPDTLGL NTRLYKWIPQ NDLLGHPKTR AFITHGGANG IYEAIYHGIP MVGVPLFADQ PDNIAHMKAK GAAVSLDFHT MSSTDLLNAL KTVINDPLYK ENAMKLSRIH HDQPVKPLDR AVFWIEFVMR HKGAKHLRVA A; Positive Samples: 22Rv1, B cells, HT-1080, Mouse liver, Mouse brain, Mouse kidney, Rat kidney; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.84 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references In Vitro Hepatic Assessment of Cineole and Its Derivatives in Common Brushtail Possums (Trichosurus vulpecula) and Rodents.
Chand RR, Nimick M, Cridge B, Rosengren RJ
Biology 2021 Dec 15;10(12)
Biology 2021 Dec 15;10(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UGT2B4 in extracts of various cell lines using UGT2B4 Polyclonal Antibody (Product # PA5-92155) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Hepatic p -nitrophenol glucuronidation activity and protein levels in mouse, rat, and possum : ( A ) Hepatic microsomes from each species were incubated with 0.1 M phosphate buffer and
