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LearnNBP1-78159
antibody from Novus Biologicals
Targeting: LDLR
LDLCQ2
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Immunoelectron microscopy Radioimmunoassay Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-78159 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78159, RRID:AB_11016939
- Product name
- Mouse Monoclonal LDLR Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Rat, Bovine, Porcine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Familial hypercholesterolemia class II low-density lipoprotein receptor response to statin treatment.
Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex.
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.
HCV derived from sera of HCV-infected patients induces pro-fibrotic effects in human primary fibroblasts by activating GLI2.
Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum.
The complex of the insect LDL receptor homolog, lipophorin receptor, LpR, and its lipoprotein ligand does not dissociate under endosomal conditions.
Regulation of ApoB secretion by the low density lipoprotein receptor requires exit from the endoplasmic reticulum and interaction with ApoE or ApoB.
Binding characteristics of a panel of monoclonal antibodies against the ligand binding domain of the human LDLr.
Overexpression of PCSK9 accelerates the degradation of the LDLR in a post-endoplasmic reticulum compartment.
Three lipoprotein receptors and cholesterol in inclusion-body myositis muscle.
Endoplasmic reticulum localization of the low density lipoprotein receptor mediates presecretory degradation of apolipoprotein B.
Grp78 is involved in retention of mutant low density lipoprotein receptor protein in the endoplasmic reticulum.
Distinct saturable pathways for the endocytosis of different tyrosine motifs.
Distinct saturable pathways for the endocytosis of different tyrosine motifs.
Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery.
Apolipoprotein E uptake and degradation via chloroquine-sensitive pathway in cultivated monkey cells overexpressing low density lipoprotein receptor.
Members of the low density lipoprotein receptor family mediate cell entry of a minor-group common cold virus.
Deletion in the first cysteine-rich repeat of low density lipoprotein receptor impairs its transport but not lipoprotein binding in fibroblasts from a subject with familial hypercholesterolemia.
Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia.
Omer L, Hindi L, Militello G, Stivers KB, Tien KC, Boyd NL
Disease models & mechanisms 2020 Apr 6;13(4)
Disease models & mechanisms 2020 Apr 6;13(4)
Sigma-2 Receptor/TMEM97 and PGRMC-1 Increase the Rate of Internalization of LDL by LDL Receptor through the Formation of a Ternary Complex.
Riad A, Zeng C, Weng CC, Winters H, Xu K, Makvandi M, Metz T, Carlin S, Mach RH
Scientific reports 2018 Nov 15;8(1):16845
Scientific reports 2018 Nov 15;8(1):16845
Efficient Generation of Somatic Cell Nuclear Transfer-Competent Porcine Cells with Mutated Alleles at Multiple Target Loci by Using CRISPR/Cas9 Combined with Targeted Toxin-Based Selection System.
Sato M, Miyoshi K, Nakamura S, Ohtsuka M, Sakurai T, Watanabe S, Kawaguchi H, Tanimoto A
International journal of molecular sciences 2017 Dec 4;18(12)
International journal of molecular sciences 2017 Dec 4;18(12)
Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.
Chen HC, Chen PY, Wu MJ, Tai MH, Yen JH
PloS one 2016;11(9):e0162414
PloS one 2016;11(9):e0162414
HCV derived from sera of HCV-infected patients induces pro-fibrotic effects in human primary fibroblasts by activating GLI2.
Granato M, Zompetta C, Vescarelli E, Rizzello C, Cardi A, Valia S, Antonelli G, Marchese C, Torrisi MR, Faggioni A, Cirone M
Scientific reports 2016 Aug 1;6:30649
Scientific reports 2016 Aug 1;6:30649
Calcium as a crucial cofactor for low density lipoprotein receptor folding in the endoplasmic reticulum.
Pena F, Jansens A, van Zadelhoff G, Braakman I
The Journal of biological chemistry 2010 Mar 19;285(12):8656-64
The Journal of biological chemistry 2010 Mar 19;285(12):8656-64
The complex of the insect LDL receptor homolog, lipophorin receptor, LpR, and its lipoprotein ligand does not dissociate under endosomal conditions.
Roosendaal SD, Kerver J, Schipper M, Rodenburg KW, Van der Horst DJ
The FEBS journal 2008 Apr;275(8):1751-66
The FEBS journal 2008 Apr;275(8):1751-66
Regulation of ApoB secretion by the low density lipoprotein receptor requires exit from the endoplasmic reticulum and interaction with ApoE or ApoB.
Blasiole DA, Oler AT, Attie AD
The Journal of biological chemistry 2008 Apr 25;283(17):11374-81
The Journal of biological chemistry 2008 Apr 25;283(17):11374-81
Binding characteristics of a panel of monoclonal antibodies against the ligand binding domain of the human LDLr.
Nguyen AT, Hirama T, Chauhan V, Mackenzie R, Milne R
Journal of lipid research 2006 Jul;47(7):1399-405
Journal of lipid research 2006 Jul;47(7):1399-405
Overexpression of PCSK9 accelerates the degradation of the LDLR in a post-endoplasmic reticulum compartment.
Maxwell KN, Fisher EA, Breslow JL
Proceedings of the National Academy of Sciences of the United States of America 2005 Feb 8;102(6):2069-74
Proceedings of the National Academy of Sciences of the United States of America 2005 Feb 8;102(6):2069-74
Three lipoprotein receptors and cholesterol in inclusion-body myositis muscle.
Jaworska-Wilczynska M, Wilczynski GM, Engel WK, Strickland DK, Weisgraber KH, Askanas V
Neurology 2002 Feb 12;58(3):438-45
Neurology 2002 Feb 12;58(3):438-45
Endoplasmic reticulum localization of the low density lipoprotein receptor mediates presecretory degradation of apolipoprotein B.
Gillian-Daniel DL, Bates PW, Tebon A, Attie AD
Proceedings of the National Academy of Sciences of the United States of America 2002 Apr 2;99(7):4337-42
Proceedings of the National Academy of Sciences of the United States of America 2002 Apr 2;99(7):4337-42
Grp78 is involved in retention of mutant low density lipoprotein receptor protein in the endoplasmic reticulum.
Jørgensen MM, Jensen ON, Holst HU, Hansen JJ, Corydon TJ, Bross P, Bolund L, Gregersen N
The Journal of biological chemistry 2000 Oct 27;275(43):33861-8
The Journal of biological chemistry 2000 Oct 27;275(43):33861-8
Distinct saturable pathways for the endocytosis of different tyrosine motifs.
Warren RA, Green FA, Stenberg PE, Enns CA
The Journal of biological chemistry 1998 Jul 3;273(27):17056-63
The Journal of biological chemistry 1998 Jul 3;273(27):17056-63
Distinct saturable pathways for the endocytosis of different tyrosine motifs.
Warren RA, Green FA, Stenberg PE, Enns CA
The Journal of biological chemistry 1998 Jul 3;273(27):17056-63
The Journal of biological chemistry 1998 Jul 3;273(27):17056-63
Generation of targeted retroviral vectors by using single-chain variable fragment: an approach to in vivo gene delivery.
Somia NV, Zoppé M, Verma IM
Proceedings of the National Academy of Sciences of the United States of America 1995 Aug 1;92(16):7570-4
Proceedings of the National Academy of Sciences of the United States of America 1995 Aug 1;92(16):7570-4
Apolipoprotein E uptake and degradation via chloroquine-sensitive pathway in cultivated monkey cells overexpressing low density lipoprotein receptor.
Jensen TG, Roses AD, Jørgensen AL
Neuroscience letters 1994 Oct 24;180(2):193-6
Neuroscience letters 1994 Oct 24;180(2):193-6
Members of the low density lipoprotein receptor family mediate cell entry of a minor-group common cold virus.
Hofer F, Gruenberger M, Kowalski H, Machat H, Huettinger M, Kuechler E, Blaas D
Proceedings of the National Academy of Sciences of the United States of America 1994 Mar 1;91(5):1839-42
Proceedings of the National Academy of Sciences of the United States of America 1994 Mar 1;91(5):1839-42
Deletion in the first cysteine-rich repeat of low density lipoprotein receptor impairs its transport but not lipoprotein binding in fibroblasts from a subject with familial hypercholesterolemia.
Leitersdorf E, Hobbs HH, Fourie AM, Jacobs M, van der Westhuyzen DR, Coetzee GA
Proceedings of the National Academy of Sciences of the United States of America 1988 Nov;85(21):7912-6
Proceedings of the National Academy of Sciences of the United States of America 1988 Nov;85(21):7912-6
Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia.
Beisiegel U, Schneider WJ, Goldstein JL, Anderson RG, Brown MS
The Journal of biological chemistry 1981 Nov 25;256(22):11923-31
The Journal of biological chemistry 1981 Nov 25;256(22):11923-31
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LDL R Antibody (C7) [NBP1-78159] - Total protein from human HeLa and HepG2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-LDL receptor in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: LDL R Antibody (C7) [NBP1-78159] - Analysis of an FFPE tissue section of human liver using LDL Receptor antibody (clone C7) with HRP-DAB based detection and hematoxylin counterstaining.
