Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-76974 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LECT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Isolation of mouse ovarian follicles for single-cell RNA-seq and in vitro culture.
Obesity modulates cell-cell interactions during ovarian folliculogenesis.
Qian J, Zhu R, Yan R, Long X, Guo F
STAR protocols 2022 Sep 16;3(3):101537
STAR protocols 2022 Sep 16;3(3):101537
Obesity modulates cell-cell interactions during ovarian folliculogenesis.
Long X, Yang Q, Qian J, Yao H, Yan R, Cheng X, Zhang Q, Gu C, Gao F, Wang H, Zhang L, Guo F
iScience 2022 Jan 21;25(1):103627
iScience 2022 Jan 21;25(1):103627
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LECT1 in extracts of mouse liver. Samples were incubated with LECT1 polyclonal antibody (Product # PA5-76974).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of LECT1 in A549 cells. The samples were probed with a LECT1 polyclonal antibody (Product # PA5-77013) followed by DAPI to stain cell nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of LECT1 in A549 cells. Samples were incubated with LECT1 polyclonal antibody (Product # PA5-76974) followed by DAPI (nuclear staining).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of LECT1 in A549 cells. Samples were incubated with LECT1 polyclonal antibody (Product # PA5-76974) followed by DAPI (nuclear staining).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Characterization of in vitro cultured granulosa cells Cultured primary granulosa cells are stained for the granulosa cell marker Cnmd, verifying the purity of isolated cells. Scale bar, 20 mum. Adapted with permission from ().