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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- PA5-78241 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAFK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Jurkat Predicted Reactivity: Mouse (98%), Rat (97%), Zebrafish (83%), Chicken (93%), Bovine (92%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAFK in Jurkat cells using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with MAFK polyclonal antibody (Product # PA5-78241) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFK Polyclonal Antibody detects MAFK protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract.15 % SDS-PAGE. MAFK Polyclonal Antibody (Product # PA5-78241) dilution: 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin immunoprecipitation analysis of MAFK in HepG2 chromatin extract using 5 µg of sample. Immunoprecipitation was performed using MAFK polyclonal antibody (Product # PA5-78241). Analysis of the precipitated DNA was done by PCR using primer targeting IL-8 or GAPDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HepG2 chromatin extract treated with LPS (10 ng/mL for 1 h) and 5 µg of either normal rabbit IgG or MAFK Polyclonal Antibody (Product # PA5-78241). The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MAFK Polyclonal Antibody immunoprecipitates MAFK protein in IP experiments. IP samples: Jurkat whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of MAFK protein by 4 µg MAFK Polyclonal Antibody (Product # PA5-78241). 15 % SDS-PAGE. The immunoprecipitated MAFK protein was detected by MAFK Polyclonal Antibody (Product # PA5-78241) diluted at 1:500.
