Western blot analysis of extracts of various cell lines, using KRAS+HRAS+NRAS rabbit monoclonal antibody (STJ11101782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using KRAS+HRAS+NRAS rabbit monoclonal antibody (STJ11101782) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunofluorescence analysis of NIH-3T3 cells using KRAS+HRAS+NRAS rabbit monoclonal antibody (STJ11101782) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600ug extracts of mouse brain using 3ug KRAS+HRAS+NRAS antibody (STJ11101782). Western blot was performed from the immunoprecipitate using KRAS+HRAS+NRAS antibody (STJ11101782) at a dilition of 1:1000.
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Western blot
Immunocytochemistry
