Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 17-1128-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD112 (Nectin-2) Monoclonal Antibody (R2.477), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This R2.477 monoclonal antibody reacts with human CD112 (also known as Nectin-2 or Poliovirus Receptor Related 2), a 60-65 kDa immunoglobulin-like transmembrane cell adhesion molecule. This cell surface molecule is expressed on hematopoietic cells, endothelial, epithelial, as well as tumor cell lines such as Jurkat, U937, and MCF7. CD112 is a ligand for DNAM-1 (CD226), a leukocyte adhesion molecule expressed on natural killer (NK) cells. Interaction between CD112 and DNAM-1 promotes NK cytotoxic activity, leading to tumor cell lysis. In addition to its role in tumor suppression, CD112 plays a cooperative role in cell-cell adhesion, particularly in the formation of adherens junctions between neighboring epithelial cells and fibroblasts. CD112 can form homophilic and heterophilic interactions to regulate contact inhibition of cell movement and proliferation. Finally, CD112 has been reported to be a receptor for herpesvirus entry into cells. This monoclonal antibody has been reported to block cell adhesion and herpesvirus entry. Applications Reported: This R2.477 antibody has been reported for use in flow cytometric analysis. Applications Tested: This R2.477 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- R2.477
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.
Nectin-3 (CD113) interacts with Nectin-2 (CD112) to promote lymphocyte transendothelial migration.
Crystal structure of cell adhesion molecule nectin-2/CD112 and its binding to immune receptor DNAM-1/CD226.
Nectins and nectin-like molecules: roles in cell adhesion, polarization, movement, and proliferation.
Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction.
The human poliovirus receptor related 2 protein is a new hematopoietic/endothelial homophilic adhesion molecule.
Catakovic K, Gassner FJ, Ratswohl C, Zaborsky N, Rebhandl S, Schubert M, Steiner M, Gutjahr JC, Pleyer L, Egle A, Hartmann TN, Greil R, Geisberger R
Oncoimmunology 2017;7(1):e1371399
Oncoimmunology 2017;7(1):e1371399
Nectin-3 (CD113) interacts with Nectin-2 (CD112) to promote lymphocyte transendothelial migration.
Devilard E, Xerri L, Dubreuil P, Lopez M, Reymond N
PloS one 2013;8(10):e77424
PloS one 2013;8(10):e77424
Crystal structure of cell adhesion molecule nectin-2/CD112 and its binding to immune receptor DNAM-1/CD226.
Liu J, Qian X, Chen Z, Xu X, Gao F, Zhang S, Zhang R, Qi J, Gao GF, Yan J
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 1;188(11):5511-20
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 1;188(11):5511-20
Nectins and nectin-like molecules: roles in cell adhesion, polarization, movement, and proliferation.
Ogita H, Takai Y
IUBMB life 2006 May-Jun;58(5-6):334-43
IUBMB life 2006 May-Jun;58(5-6):334-43
Expression of the DNAM-1 ligands, Nectin-2 (CD112) and poliovirus receptor (CD155), on dendritic cells: relevance for natural killer-dendritic cell interaction.
Pende D, Castriconi R, Romagnani P, Spaggiari GM, Marcenaro S, Dondero A, Lazzeri E, Lasagni L, Martini S, Rivera P, Capobianco A, Moretta L, Moretta A, Bottino C
Blood 2006 Mar 1;107(5):2030-6
Blood 2006 Mar 1;107(5):2030-6
The human poliovirus receptor related 2 protein is a new hematopoietic/endothelial homophilic adhesion molecule.
Lopez M, Aoubala M, Jordier F, Isnardon D, Gomez S, Dubreuil P
Blood 1998 Dec 15;92(12):4602-11
Blood 1998 Dec 15;92(12):4602-11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD11c FITC (Product # 11-0116-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD112 APC (right). Cells in the monocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL