Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-77525 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin 2 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate(lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6) with Nectin 2 (extracellular) polyclonal antibody (Product # PA5-77525) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat spleen lysate (lanes 1 and 4), human Jurkat T cell leukemia cell lysate(lanes 2 and 5) and human K562 erythroleukemia cell lysate (lanes 3 and 6) with Nectin 2 (extracellular) polyclonal antibody (Product # PA5-77525) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Nectin 2 (extracellular) in perfusion-fixed, frozen rat brain. Samples were probed with Nectin 2 (extracellular) polyclonal antibody (Product # PA5-77525) using a dilution of 1:600, and incubated with goat anti-rabbit Alexa Fluor 488 and DAPI. Nectin-2 staining (green) appears in in apical dendrites of CA1 pyramidal neurons (vertical arrows) and in astrocytes (horizontal arrows).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of human Jurkat T cells leukemia cellss using polyclonal Nectin 2 (extracellular) antibody (Product # PA5-77525) with a dilution of 1:15 followed by a goat-anti-rabbit-Alexa-488 secondary antibody.