MA5-34802

antibody from Invitrogen Antibodies

Targeting: NECTIN2 CD112, HVEB, PRR2, PVRL2, PVRR2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34802

Provider

Invitrogen Antibodies

Product name

Nectin 2 Recombinant Rabbit Monoclonal Antibody (JG39-64)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: K562, MCF-7, SK-OV-3, LOVO, rat testis tissue, human liver cancer tissue, mouse testis tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG39-64

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles, store in dark

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nectin 2 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nectin 2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nectin 2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nectin 2 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nectin 2 in paraffin-embedded human liver cancer tissue. Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nectin 2 in paraffin-embedded rat testis tissue. Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nectin 2 in paraffin-embedded mouse testis tissue. Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Nectin 2 in LOVO cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Nectin 2 in LOVO cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.