MA5-34802

NECTIN2 antibody from Invitrogen Antibodies

CD112, HVEB, PRR2, PVRL2, PVRR2

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

Data presented on provider website

Flow cytometry

Supportive data in Antibodypedia

Provider product page for MA5-34802

Antibody Data

Product number

MA5-34802

Provider

Invitrogen Antibodies

Product name

Anti-Nectin 2 Recombinant Rabbit Monoclonal Antibody (JG39-64)

Provider product page

Antibody type

Other

Antigen

Recombinant full-length protein

Description

Positive Control: K562, MCF-7, SK-OV-3, LOVO, rat testis tissue, human liver cancer tissue, mouse testis tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JG39-64

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nectin 2 in K562 (1), MCF-7 (2) and SK-OV-3 (3) cells lysates. Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nectin 2 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Nectin 2 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Nectin 2 monoclonal antibody (Product # MA5-34802), followed by DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Nectin 2 in LOVO cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Nectin 2 monoclonal antibody (Product # MA5-34802) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.