A08081-2

NECTIN2 antibody from Boster Biological Technology

CD112, HVEB, PRR2, PVRL2, PVRR2

Western blot

Supportive data in Antibodypedia

ELISA

Data presented on provider website

Immunocytochemistry

Data presented on provider website

Immunohistochemistry

Data presented on provider website

Flow cytometry

Data presented on provider website

Provider product page for A08081-2

Antibody Data

Product number

A08081-2

Provider

Boster Biological Technology

Product name

Anti-Nectin 2/NECTIN2 Antibody Picoband™

Provider product page

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Nectin 2/NECTIN2 detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Rat.

Reactivity

Human, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Nectin 2/NECTIN2 using anti-Nectin 2/NECTIN2 antibody (A08081-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin 2/NECTIN2 antigen affinity purified polyclonal antibody (Catalog # A08081-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin 2/NECTIN2 at approximately 70-80KD. The expected band size for Nectin 2/NECTIN2 is at 58KD.

Additional image