Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP2-24535 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal OLFM4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A fusion protein composed of the DSL domain of Dll1 and RGD motif protects cryptic stem cells in irradiation injury.
Plasma olfactomedin 4 level in peripheral blood and its association with clinical features of breast cancer.
Autophagy is active in normal colon mucosa.
Olfactomedin-4 regulation by estrogen in the human endometrium requires epidermal growth factor signaling.
Sun ZJ, Zhang YZ, Liu F, Chen JJ, Chen DX, Liu HB, Liang L, Han H
Bioscience reports 2018 Apr 27;38(2)
Bioscience reports 2018 Apr 27;38(2)
Plasma olfactomedin 4 level in peripheral blood and its association with clinical features of breast cancer.
Liu C, Guo Y, Wu W, Zhang Z, Xu L, Wu K, Hu W, Liu G, Shi J, Xu C, Bi J, Sheng Y
Oncology letters 2017 Dec;14(6):8106-8113
Oncology letters 2017 Dec;14(6):8106-8113
Autophagy is active in normal colon mucosa.
Groulx JF, Khalfaoui T, Benoit YD, Bernatchez G, Carrier JC, Basora N, Beaulieu JF
Autophagy 2012 Jun;8(6):893-902
Autophagy 2012 Jun;8(6):893-902
Olfactomedin-4 regulation by estrogen in the human endometrium requires epidermal growth factor signaling.
Dassen H, Punyadeera C, Delvoux B, Schulkens I, Marchetti C, Kamps R, Klomp J, Dijcks F, de Goeij A, D'Hooghe T, Kyama C, Ederveen A, Dunselman G, Groothuis P, Romano A
The American journal of pathology 2010 Nov;177(5):2495-508
The American journal of pathology 2010 Nov;177(5):2495-508
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: OLFM4 Antibody [NBP2-24535] - Analysis of human OLFM4 in human liver lysate in the 1) absence and 2) presence of immunizing peptide using OLFM4 antibody at 2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: OLFM4 Antibody [NBP2-24535] - Simple Western lane view shows a specific band for OLMF4 in 0.2 mg/ml of Human Liver (left) and Mouse Liver (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Analysis of OLFM4 in formalin-fixed, paraffin-embedded human liver tissue using OLFM4 antibody at 5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse stomach using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated very specific staining for OLFM4 in almost all cell types. The signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm. The simple columnar epithelial cells showed stronger and a distinct inter-cellular signal compared to the cells of the gastric glands.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-24535] - Tissue section of mouse liver using OLFM4 antibody at 2.5ug/ml concentration with HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice staining in all the hepatocytes and the signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm.