PA5-73003

antibody from Invitrogen Antibodies

Targeting: OLFM4 GC1, GW112, OlfD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-73003

Provider

Invitrogen Antibodies

Product name

OLFM4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OLFM4 in human liver (1: no immunizing peptide; 2: with immunizing peptide) tissue lysate using OLFM4 polyclonal antibody (Product # PA5-73003) at a dilution on 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OLFM4 in human liver lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in OLFM4 polyclonal antibody (Product # PA5-73003) using a dilution of 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OLFM4 in 0.2 mg/mL OLFM4. Samples were incubated in OLFM4 polyclonal antibody (Product # PA5-73003). This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of OLFM4 in Tissue section of mouse stomach. Samples were incubated in OLFM4 polyclonal antibody (Product # PA5-73003) using a dilution of 2.5 µg/mL followed by HRP-DAB detection and hematoxylin counterstaining. The antibody generated very specific staining for OLFM4 in almost all cell types. The signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm. The simple columnar epithelial cells showed stronger and a distinct inter-cellular signal compared to the cells of the gastric glands.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of OLFM4 in Tissue section of mouse liver. Samples were incubated in OLFM4 polyclonal antibody (Product # PA5-73003) using a dilution of 2.5 µg/mL followed by HRP-DAB detection and hematoxylin counterstaining. The antibody generated nice staining in all the hepatocytes and the signal was localized specifically to the inter-cellular spaces, the nuclei and the cytoplasm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of OLFM4 in formalin-fixed, paraffin-embedded human liver tissue. Samples were incubated in OLFM4 polyclonal antibody (Product # PA5-73003) using a dilution of 5 µg/mL.