Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-57529 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OLFM4 Recombinant Rabbit Monoclonal Antibody (24GB680)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 24GB680
- Vial size
- 20 µL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OLFM4. Total cell lysates (30 μg) from C2C12, H9c2, HAP-1, HeLa, HepG2, and HT-1080 cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OLFM4 Recombinant Monoclonal Antibody (Product # MA5-57528) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of OLFM4. Olfactomedin 4 expression in wild type (WT) and Olfactomedin 4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 alpha serves as a loading control. The blot was incubated with OLFM4 Recombinant Monoclonal Antibody (Product # MA5-57528) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical staining of OLFM4 in C2C12 cells using OLFM4 Recombinant Monoclonal Antibody (Product # MA5-57528) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; Olfactomedin 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of OLFM4 expression in C2C12 cells using OLFM4 Recombinant Monoclonal Antibody (Product # MA5-57528) at a 1:2,000 dilution. Green, isotype control; red, Olfactomedin 4.
