Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AGR-088-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR108 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse TK-1 T-cell lymphoma cell line lysate (lanes 1 and 4), human Jurkat T-cell leukemia cell line lysate (lanes 2 and 5) and mouse BV-2 microglia cell line lysate (lanes 3 and 6): - 1-3. Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200). 4-6. Anti-GPR108 (extracellular) Antibody , preincubated with GPR108 (extracellular) Blocking Peptide (BLP-GR088).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1-2. Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200). 3-4. Anti-GPR108 (extracellular) Antibody , preincubated with GPR108 (extracellular) Blocking Peptide (BLP-GR088).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of GPR108 in mouse red nucleus. Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GPR108 (extracellular) Antibody (#AGR-088), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR108 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the Antibody with GPR108 (extracellular) Blocking Peptide (BLP-GR088), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of GPR108 by indirect flow cytometry in live intact mouse BV-2 microglia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-GPR108 (extracellular) Antibody (#AGR-088), (2.5μg) + goat- Anti-rabbit-FITC.