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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-46991 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL15RA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 10% cross-reactivity with recombinant human IL-2 R gamma is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IL15RA in immersion fixed HDLM‚2 human Hodgkins lymphoma cell line. Samples were incubated in IL15RA polyclonal antibody (Product # PA5-46991) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IL15RA in immersion fixed paraffin-embedded sections of human prostate cancer tissue. Samples were incubated in IL15RA polyclonal antibody (Product # PA5-46991) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL15RA is shown by the increase in mean CPM (count per minute- counted radioactivity- measure of cell proliferation) with increasing concentrations of IL15RA Polyclonal Antibody (PA5-46991).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL15RA in CTLL‚2 mouse cytotoxic T cell line. Samples were incubated in IL15RA polyclonal antibody (Product # PA5-46991). Recombinant Human IL‚15 Rα Fc Chimera inhibits Recombinant Human IL‚15 induced proliferation in the CTLL‚2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‚15 (2 ng/mL) activity elicited by Recombinant Human IL‚15 Rα Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚15 Rα Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1‚4 µg/mL.
