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LearnPA5-46991
antibody from Invitrogen Antibodies
Targeting: IL15RA
CD215, IL-15RA
Western blot
Immunocytochemistry
Immunohistochemistry Flow cytometry Blocking/Neutralizing Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-46991 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL15RA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 10% cross-reactivity with recombinant human IL-2 R gamma is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IL15RA in immersion fixed HDLM‚2 human Hodgkins lymphoma cell line. Samples were incubated in IL15RA polyclonal antibody (Product # PA5-46991) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of IL15RA is shown by the increase in mean CPM (count per minute- counted radioactivity- measure of cell proliferation) with increasing concentrations of IL15RA Polyclonal Antibody (PA5-46991).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL15RA in CTLL‚2 mouse cytotoxic T cell line. Samples were incubated in IL15RA polyclonal antibody (Product # PA5-46991). Recombinant Human IL‚15 Rα Fc Chimera inhibits Recombinant Human IL‚15 induced proliferation in the CTLL‚2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‚15 (2 ng/mL) activity elicited by Recombinant Human IL‚15 Rα Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚15 Rα Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 1‚4 µg/mL.
