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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- 14-7159-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD215 (IL-15Ra) Monoclonal Antibody (eBioJM7A4), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioJM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL-2Rbeta and gamma chains, which are essential for signal transduction. The IL-15Ralpha receptor is structurally related to IL-2Ralpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2Ralpha and IL-2. In the human, 3 differentially spliced human IL-15Ralpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL-15Ralpha, like that of IL-2Ralpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL-2Rbeta and IL-2Rgamma in the absence of the alpha subunit.
- Antibody clone number
- eBioJM7A4
- Concentration
- 0.5 mg/mL
Submitted references Natural killer cells from children with type 1 diabetes have defects in NKG2D-dependent function and signaling.
IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells.
IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells.
Functional characterization of the human interleukin-15 receptor alpha chain and close linkage of IL15RA and IL2RA genes.
Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha chain of the IL-2 receptor.
Qin H, Lee IF, Panagiotopoulos C, Wang X, Chu AD, Utz PJ, Priatel JJ, Tan R
Diabetes 2011 Mar;60(3):857-66
Diabetes 2011 Mar;60(3):857-66
IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells.
Vámosi G, Bodnár A, Vereb G, Jenei A, Goldman CK, Langowski J, Tóth K, Mátyus L, Szöllösi J, Waldmann TA, Damjanovich S
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11082-7
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11082-7
IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells.
Dubois S, Mariner J, Waldmann TA, Tagaya Y
Immunity 2002 Nov;17(5):537-47
Immunity 2002 Nov;17(5):537-47
Functional characterization of the human interleukin-15 receptor alpha chain and close linkage of IL15RA and IL2RA genes.
Anderson DM, Kumaki S, Ahdieh M, Bertles J, Tometsko M, Loomis A, Giri J, Copeland NG, Gilbert DJ, Jenkins NA
The Journal of biological chemistry 1995 Dec 15;270(50):29862-9
The Journal of biological chemistry 1995 Dec 15;270(50):29862-9
Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha chain of the IL-2 receptor.
Giri JG, Kumaki S, Ahdieh M, Friend DJ, Loomis A, Shanebeck K, DuBose R, Cosman D, Park LS, Anderson DM
The EMBO journal 1995 Aug 1;14(15):3654-63
The EMBO journal 1995 Aug 1;14(15):3654-63
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of adherent human PBMCs stimulated with LPS and IFN gamma for 24 hours with 0.25 µg of Mouse IgG2b K Isotype Control Purified (Product # 14-4732-82) (open histogram) or 0.25 µg of Anti-Human CD215 (IL-15 Receptor alpha) Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87).Cells in the large scatter population were used for analysis.
